트리스(하이드록시메틸)아미노메탄

트리스(하이드록시메틸)아미노메탄 속성

트리스(하이드록시메틸)아미노메탄 MSDS

2-Amino-2-(hydroxymethyl)-1,3-propanediol

트리스(하이드록시메틸)아미노메탄 C화학적 특성, 용도, 생산

화학적 성질

White, crystalline solid. PH (0.1M aqueous solution) 10.6, corrosive to copper, brass, aluminum. Solubility in water 80 g/100 cc (20C). Combustible.

용도

Tris base has been used:

• In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis
• As a component of sample buffer for SDS-PAGE
• In the preparation of IEC (ion-exchange chromatography) buffer

주요 응용

Tris(hydroxymethyl)aminomethane is mainly required for preparation of buffers at physiological range of 7.3 to 7.5. The prepared buffers are compatible with biological fluids. It is of importance to laboratories as a standard pH solution.
Tris(hydroxymethyl)aminomethane has been used as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.
Tris(hydroxymethyl)aminomethane is used as a buffer in biochemistry and molecular biology laboratories. It is used as a primary standard to standardize acid solutions for chemical analysis. It finds application in cell membranes to increase permeability. As an alternative to sodium bicarbonate, it used in the treatment of metabolic acidosis. It acts as a precursor for the preparation of polymers, oxazolones and oxazolidines.

제조 방법

Tris is prepared industrially by the exhaustive condensation of nitromethane with formaldehyde under basic conditions (i.e. repeated Henry reactions) to produce the intermediate (HOCH2)3CNO2, which is subsequently hydrogenated to give the final product.

일반 설명

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Purification Methods

TRIS can ordinarily be obtained in highly pure form suitable for use as an acidimetric standard. If only impure material is available, it should be crystallised from 20% EtOH, aqueous MeOH (m 171.1o) or isopropanol (m 172-173o). Dry it in a vacuum desiccator over P2O5 or CaCl2. Alternatively, it is dissolved in twice its weight of water at 55-60o, filtered, concentrated to half its volume and poured slowly, with stirring, into about twice its volume of EtOH. The crystals which separate on cooling to 3-4o are filtered off, washed with a little MeOH, air dried by suction, then finally ground and dried in a vacuum desiccator over P2O5. It has also been recrystallised from water, MeOH or aqueous MeOH, and vacuum dried at 80o for 2 days. [Beilstein 4 H 303, 4 III 857, 4 IV 1903.]

트리스(하이드록시메틸)아미노메탄 준비 용품 및 원자재

원자재

준비 용품

트리스(하이드록시메틸)아미노메탄 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Suzhou Genemill Biotechnology Co.,Ltd	+8615862502491	aimeegao@genemill.com	China	38	58
Jinan Qinmu Fine Chemical Co.,Ltd.	+8618660799346	sales@qinmuchem.com	China	1009	58
Hubei Xindesheng Material Technology Co., Ltd.	+8618971041571	vickyzhao@whdschem.com	China	169	58
Beijing Risun Science and Technology Limited	+86-01063701806 +86-19801193650	xykj@risun.com	China	15	58
Hefei TNJ Chemical Industry Co.,Ltd.	0551-65418684 +8618949823763	sales@tnjchem.com	China	25363	58
Zouping mingyuan import & export trading co., ltd	+86-0543-2240078 +8618364991597	myzhao793@163.com	China	994	58
XuZhou Magic Biotechnology Co., Ltd.	+undefined13921770081	SVP01@magicbiotech.cn	China	86	58
Henan Allgreen Chemical Co.,LTD	+86-37155567971 +86-13633837469	info@allgreenchem.com	China	5985	58
Henan Bao Enluo International TradeCo.，LTD	+86-17331933971 +86-17331933971	deasea125996@gmail.com	China	2180	58
Hebei Yibangte Import and Export Co. , Ltd.	+86-0311-85030958 +8615532196582	lisa@yibangte.com	China	3023	58

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